SUGARCANE GENOMICS AND BIOTECHNOLOGY: STATE OF THE ART, CHALLENGES AND ACTIONS

Souza, Glaucia Mendes; Sluys, Marie-Anne Van;

Resumo:

Sugarcane belongs to the botanic group known as Saccharum officianale, domesticated since 7 000 BC. The process of domestication started in New Guinea from where seedlings were taken to Continental Asia. In China and India, prevailing favorable conditions allowed the development of two different species, S. sinense and S. barberi respectively. From approximately a century ago, selective breeding produced Saccharum officinarum, also known as Nobel Clones. These varieties have been progressively replaced by modern varieties from crossings between S. officinarum and S. robustum,species which currently predominate worldwide. As sugarcane is a hybrid plant, the identification of genes of greater economic interest is a laborious and lengthy process e.g. it takes about 15 years to release a new commercial variety. This gives an indication of the difficulties involved in developing new commercial varieties. In Brazil, increasing commercial pressure to improve sugarcane productivity faces the challenge to develop new varieties adapted to the different conditions in the country. For example, it is estimated that to supply internal and external demand, Brazil will have to duplicate sugarcane production within the next 5 to 7 years, requiring investment in new mills, expansion of cultivated area, increase productivity and better cane varieties etc.

Part 3 — A New model for sugarcane mechanization system :

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DOI: 10.5151/BlucherOA-Sugarcane-SUGARCANEBIOETHANOL_32

Referências bibliográficas
  • 1. VETTORE, A. L.; SILVA, F. R. da; KEMPER, E. L.; SOUZA, G. M.; SILVA, A. M. da; FERRO, M. I.; HENRIQUE-SILVA, F.; GIGLIOTI, E. A.; LEMOS, M. V.; COUTINHO, L. L. et al. Analysis and functional annotation of an expressed sequence tag collection for tropical crop sugarcane. Genome research, 2003, 13(12):2725-2735.
    2. VINCENTZ, M.; CARA, F. A.; OKURA, V. K.; SILVA, F. R.; PEDROSA, G. L.; HEMERLY, A. S.; CAPELLA, A. N.; MARINS, M.; FERREIRA, P. C.; FRANCA, S. C. et al. Evaluation of monocot and eudicot divergence using the sugarcane transcriptome. Plant physiol., 2004, 134(3):951-959.
    3. ARRUDA, P. Sugarcane transcriptome. A landmark in plant genomics in the tropics. Genetic and molecular biology, 2001, 24:1-4.
    4. PAPINI-TERZI, F. S.; FELIX, J. M.; ROCHA, F. R.; WACLAWOVSKY, A. J.; ULIAN, E. C.; CHABREGAS, S.; FALCO, M. C.; NISHIYAMA-JR, M. Y.; VÊNCIO, R. Z. N.; VICENTINI, R.; MENOSSI, M.; SOUZA, G. M. The Sucest-FU/Project: identifying genes that regulate sucrose content in sugarcane plants. Proc. Int. soc. sugarcane technol, 2007, 26.
    5. SOUZA, G. M.; PAPINI-TERZI, F. S.; ROCHA, F. R.; WACLAWOVSKY, A. J.; VÊNCIO, R. Z. N.; MARQUES, J. O.; FELIX, J. M.; TEIXEIRA, M. M.; ULIAN, E. C. Sugarcane with increased sucrose levels. In: United States: Universidade de São Paulo, Universidade de Campinas, Fundação de Amparo à Pesquisa do Estado de São Paulo, Centro de Tecnologia Canavieira e Central de Álcool Lucélia, 2006.
    6. SOUZA, G. M.; PAPINI-TERZI, F. S.; ROCHA, F. R.; WACLAWOVSKY, A. J.; VÊNCIO, R. Z. N.; MARQUES, J. O.; FELIX, J. M.; TEIXEIRA, M. M.; BUCKERIDGE, M.; SOUZA, A. P.; ULIAN, E. C. Genes associated to sucrose content. In: USPTO. Universidade de São Paulo, Universidade de Campinas, Fundação de Amparo à Pesquisa do Estado de São Paulo, Centro de Tecnologia Canavieira e Central de Álcool Lucélia, 2006.
    7. SOUZA, G. M.; PAPINI-TERZI, F. S.; ROCHA, F. R.; WACLAWOVSKY, A. J.; VÊNCIO, R. Z. N.; MARQUES, J. O.; FELIX, J. M.; TEIXEIRA, M. M.; BUCKERIDGE, M.; SOUZA, A. P.; ULIAN, E. C. Genes associated to sucrose content. In: USPTO. Universidade de São Paulo, Universidade de Campinas, Fundação de Amparo à Pesquisa do Estado de São Paulo, Centro de Tecnologia Canavieira e Central de Álcool Lucélia, 2007.
    8. ROCHA, F. R.; PAPINI-TERZI, F. S.; NISHIYAMA, M. Y., JR., VENCIO R. Z.; VICENTINI, R.; DUARTE, R. D.; ROSA, V. E. de, JR., VINAGRE F., BARSALOBRES, C.; MEDEIROS, A. H. et al. Signal transduction-related responses to phytohormones and environmental challenges in sugarcane. BMC genomics, 2007, 8:71.
    9. SOUZA, G. M. Transcriptoma da cana-de-açúcar. In: Universidade de São Paulo, Universidade Estadual de Campinas: Fapesp, CTC e Centralcool, 2004.
    10. GALLO-MEAGHER, M. I. J. Herbicide resistant transgenic sugarcane plants containing the bar gene. Crop Sci, 1996, 36:1367-1374.
    11. ENRIQUEZ-OBREGON, G. A. V. P.; PRIETO, S. D. L.; RIVA-GUSTAVO, A. D. L.; SELMAN, H. G. Herbicide resistant sugarcane (Saccharum officinarum) plants by Agrobacterium-mediated transformation. Planta, 1998, 206: 20-27.
    12. FALCO, M. C. T-NA; ULIAN, E. C. Transformation and expression of a gene for herbicide resistance in a Brazilian sugarcane. Plant cell reports, 2000, 19:1188-1194.
    13. LEIBBRANDT, N. B. S. S. Stability of gene expression and agronomic performance of a transgenic herbicide-resistant sugarcane line in South Africa. Crop sci, 2003, 43:671-678.
    14. SNYMAN, S. J. M. G.; RICHARDS, J. M.; HARICHARAN, N.; RAMGAREEB, S.; HUCKETT, B. I. Refining the application of direct embryogenesis in sugarcane: effect of the developmental phase of leaf disk explants and the timing of D/A transfer on transformation efficiency. Plant cell rep., 2006, 25:1016-1023.
    15. ARENCIBIA, A. V. R.; PIETRO, D.; TELLEZ, P.; CARMONA, E. R.; COEGO, A.; HERNANDEZ, L.; DE LA RIVA GA. Transgenic sugarcane plants resistant to stem borer attack. Mol breed, 1997, 3:247-255.
    16. ARENCIBIA, A. C. E.; CORNIDE, M. T.; CASTIGLIONE, S.; O’RELLY, J.; CINEA, A.; ORAMAS, P.; SALA, F. Somaclonal variation in insect-resistant transgenic sugarcane (Saccharum hybrid) plants producd by cell electroporation. Transgenic Res., 1999, 8:349-360.
    17. FALCO, M. C. S-FM. Expression of soybean proteinase inhibitors in transgenic sugarcane plants: efects on natural defense against Diatraea saccharalis. Plant physiol. and biochem., 2003, 41:761-766.
    18. INGELBRECHT, I. L. I. J.; MIRKOV, T. E. Postranscriptional gene silencing in transgenic sugarcane. Dissection of homology dependent virus resitance in a monocot that has a complex polyploid genome. Plant physiol. and biochem., 1999, 119:1187-1197.
    19. BUTTERFIELD, M. K. I. J.; VALDEZ, GARCIA M.; MIRKOV, T. E. Inheritance and segregation of virus and herbicide resistance transgenes in sugarcane. Theor Appl. Genet., 2002, 104:797-803.
    20. MCQUALTER, R. B. D. J.; HARDING, R. H.; MCMAHON, J. A.; SMITH, G. R. Production and evaluation of transgenic sugarcane containing a Fiji disease virus (FDV) genome segment S9-derived synthetic resistance gene. Aust J Agric. Res., 2004, 55:139-145.
    21. ZHANG, L.; XU, J.; BIRCH, R. G. Engineered detoxification confers resistance against a pathogenic bacterium. Nat. biotechnol., 1999, 17(10):1021-1024.
    22. MCQUALTER, R. B.; CHONG, B. F.; MEYER, K.; VAN DYK DE, O’SHEA, M. G.; WALTON, N. J.; VIITANEN, P. V.; BRUMBLEY, S. M. Initial evaluation of sugarcane as a production platform for phydroxybenzoic acid. Plant biotechnology journal, 2005, 3(1):29-41.
    23. VICKERS, J. E. G. C.; BONNETT, G. D.; JACKSON, P. A.; KNIGHT, D. P.; ROBERTS, S. E.; ROBINSON, S. P. Overexpression of polyphenol oxidase in transgenic sugarcane results in darker juice and raw sugar. Crop sci., 2005, 45:354-362.
    24. VICKERS, J.; GROF, C.; BONNETT, G.; JACKSON, P.; MORGAN, T. Effects of tissue culture, biolistic transformation, and introduction of PPO and SPS gene constructs on performance of sugarcane clones in the field. Australian Journal of agricultural research, 2004, 56(1):57-68.
    25. VICKERS, J.E. G. C.; BONNETT, G. D.; JACKSON, P. A.; MORGAN, T. E. Effects of tissue culture, biolistic transformation, and introduction of PPO and SPS gene constructs a perfomance of sugarcane clones in the field. Aust. J. agric. res., 2005, 56:57-68.
    26. WANG, M. L.; GOLDSTEIN, C.; SU, W.; MOORE, P. H.; ALBERT, H. H. Production of biologically active MCSF in sugarcane: a secure biofactory. Transgenic res., 2005, 14(2):167-178.
    27. ZHANG, S-Z YB-P.; FENG, C-L.; CHEN, R-K.; LUO, J-P.; CAI, W-W.; LIU, F-H. Expression of the Grifola frondosa trehalose synthase gene and improvement of drought-tolerance in sugarcane (Saccharum officinarum L.). Journal of integrative plant biology, 2006, 48:453-459.
    28. MOLINARI HBM, C. J.; DAROS, E.; CAMPOS, M. K. F.; CARVALHO, J. F. R. P.; BESPALHOK FILHO, J. C.; PEREIRA, L. F. P.; VIEIRA, L. G. E. Evaluation of the tressinducible production of proline in transgenic sugarcane (Saccharum spp.): osmotic adjustment, chlorophyll fluorescence and oxidative stress. Physiologia plantarum, 2007, 130:218-229.    29. LIMA, MAC G. R.; MARTINS, G. S.; MANSUR, E. Morfogênese in vitro e susceptibilidade de calos de variedades nacionais de cana-de-açúcar (Saccharum officinarum L.) a agentes seletivos utilizados em sistemas de transformação genética. Revista Brasil Bot., 2001, 24:73-77.
    30. CIDADE, DAP G. R.; DUARTE, A. C.; SACHETTO-MARTINS, G.; MANSUR, E. Morfogênese in vitro de variedades brasileiras de cana-de-açúcar. Pesq. agropec. bras., 2006, 41:385-391.
    31. ARENCIBIA, A. D. An efficient protocol for sugarcane (Saccharum spp. L.) transformation mediated by Agrobacterium tumefaciens. Transgenic res., 1998, 7:213-222.
    32. ELLIOTT, A. R. C. J.; DUGDALE, B.; BRETELL, R. I. S.; GROF, C. P. L. Green-fluorescent protein facilitates rapid in vivo detction of genetically transformed plant cells. Plant cell rep., 1999, 18:707-714.
    33. ELLIOTT, A. R. C. J.; BRETELL, R. I. S.; GROF, C. P. L. Agrobacterium-mediatd transformation of sugarcane using GFP as a screenable marker. Aust J. plant physiol., 1998, 25:739-743.
    34. MANICKAVASAGAM, M.; GANAPATHI, A.; ANBAZHAGAN, V. R.; SUDHAKAR, B.; SELVARAJ, N.; VASUDEVAN, A.; KASTHURIRENGAN, S. Agrobacterium-mediated genetic transformation and development of herbicide-resistant sugarcane (Saccharum species hybrids) using auxiliary buds. Plant cell rep., 2004, 23(3):134-143.
    35. D’HONT, A.; GLASZMANN, J. C. Unravelling the genome structure of polyploids using FISH and GISH; examples of sugarcane and banana. Cytogenet genome res., 2005, 109(1-3):27-33.
    36. PINTO, L. R.; OLIVEIRA, K. M.; MARCONI, T.; GARCIA, A. A. F.; ULIAN, E. C.; SOUZA, A. P. de. Characterization of novel sugarcane expressed sequence tag microsatellites and their comparison with genomic SSRs. Plant breeding, 2006, 125:378-384.
    37. PINTO, L. R.; OLIVEIRA, K. M.; ULIAN, E. C.; GARCIA, A. A.; SOUZA, A. P. de. Survey in the sugarcane expressed sequence tag database (Sucest) for simple sequence repeats. Genome, 2004, 47(5):795-804.
    38. GARCIA, A. A.; KIDO, E. A.; MEZA, A. N.; SOUZA, H. M.; PINTO, L. R.; PASTINA, M. M.; LEITE, C. S.; SILVA, J. A.; ULIAN, E. C.; FIGUEIRA, A. et al. Development of an integrated genetic map of a sugarcane (Saccharum spp.) commercial cross, based on a maximum-likelihood approach for estimation of linkage and linkage phases. Theor appl genet, 2006, 112(2):298-314.
    39. OLIVEIRA KMP, L. R.; MARCONI, T. G.; MARGARIDO, G. R. A.; PASTINA, M. M.; TEIXEIRA, L. H. M.; FIGUEIRA, A. V.; ULIAN, E. C.; GARCIA, A. A. F.; SOUZA, A. P. Functional integrated genetic linkage map based on EST-markers for a sugarcane (Saccharum spp.) commercial cross. Molecular breeding, 2007, 20(3):189.
    40. PAPINI-TERZI, F. S.; ROCHA, F. R.; VENCIO, R. Z.; OLIVEIRA, K. C.; FELIX, J. M.; VICENTINI, R.; ROCHA, C. S.; SIMOES, A. C.; ULIAN, E. C.; di MAURO, S. M. et al. Transcription profiling of signal transduction-related genes in sugarcane tissues. DNA res, 2005, 12(1):27-38.
    41. SOUZA, G. M.; ROCHA, F. R.; MARQUES, J.; WACLAWOVSKI, A. J. Sequências de nucleotídeos de cana que promovem a expressão de genes. In: INPI. Universidade de São Paulo, Central de Álcool Lucélia, 2007.
    42. SILVA-FILHO MCM, D. S.; BARATA, R. M.; MEDEIROS, A. H.; POMPERMAYER, P. Promotor do gene de defesa de cana-de-açúcar induzido por herbivoria e sua utilização. In: INPI, 2005.
    43. VAN-SLUYS MAR, M.; ARAÚJO, P. G. Retroprom: método de obtenção e uso. In: INPI, 2004.
    44. BRAGA, D. P. V.; ARRIGONI, E. D. B.; BURNQUIST, W. L.; SILVA-FILHO, M. C.; ULIAN, E. C. A new approach for control of Diatraea saccharalis (Lepidoptera: Crambidae) through the expression of an insecticidal CryIa(b) protein in transgenic sugarcane. Proc. int. soc. sugarcane technol., 2001, 24(2):331-336.
    45. NUTT, K. A.; ALLSOPP, P. G.; MCGHIE, T. K.; SHEPHERD, K. M.; JOYCE, P. A. Transgenic sugarcane with increased resistance to canegrubs. Proc. austr. soc. sugarcane technol., 1999(21):171-176.
    46. WENG, L. X.; DENG, H.; XU, J. L.; LI, Q.; WANG, L. H.; JIANG, Z.; ZHANG, H. B.; LI, Q.; ZHANG, L. H. Regeneration of sugarcane elite breeding lines and engineering of stem borer resistance. Pest management science, 2006, 62(2):178-187.
    47. CHEN, P. H.; LIN, M. J.; XUE, Z. P.; CHEN, R. K. A. Study on genetic transformation of G/A gene in sugarcane. Acta agriculturae universitatis jiangxiensis, 2004, 26(5):740-743.
    48. JOYCE, P. A.; MCQUALTER, R. B.; BERNAD, M. J.; SMITH, G. R. Engineering for resistance to SCMV in sugarcane. Acta hort, 1998, 461:385-391.
    49. INGELBRECHT, I. L.; IRVINE, J. E.; MIRKOV, T. E. Posttranscriptional gene silencing in transgenic sugarcane. Dissection of homology-dependent virus resistance in a monocot that has a complex polyploid genome. Plant physiol., 1999, 119(4):1187-1198.
    50. MCQUALTER, R. B. H. R.; DALE, J. L.; SMITH, G. R. Virus derived transgenes confer resistance to Fiji disease in transgenic sugarcane plants. Proc. int. soc. sugarcane technol., 2001, 24(2):584-585.   51. BRAGA, D. P. V.; ARRIGONI, E. D. B.; SILVA-FILHO, M. C.; ULIAN, E. C. Expression of the cry1Ab protein in genetically modified sugarcane for the control of Diatraea saccharalis (Lepidoptera: Crambidae). J. new seeds, 2003, 5:209-221.
    52. SOARES-COSTA, A.; BELTRAMINI, L. M.; THIEMANN, O. H.; HENRIQUE-SILVA, F. A sugarcane cystatin: recombinant expression, purification, and antifungal activity. Biochem biophys res commu, 2002, 296:1194-1199.
    53. SOUZA, A. P.; GASPAR, M.; SILVA, E. A.; ULIAN, E.; WACLAWOVSKY, A. J.; NISHIYAMA-JR, M. Y.; VICENTINI, R.; MENOSSI, M.; SOUZA, G. M.; BUCKERIDGE, M. S. Elevated CO2 induces increases in photosynthesis, biomass, productivity and modifies gene expression in sugarcane. Plant, cell and environment.
Como citar:

SOUZA, Glaucia Mendes; SLUYS, Marie-Anne Van; "SUGARCANE GENOMICS AND BIOTECHNOLOGY: STATE OF THE ART, CHALLENGES AND ACTIONS", p. 325 -332. In: Sugarcane bioethanol — R&D for Productivity and Sustainability. São Paulo: Blucher, .
ISBN: 978-85-212-0822-8, DOI 10.5151/BlucherOA-Sugarcane-SUGARCANEBIOETHANOL_32